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The PCR conditions consisted of denaturation at 95 C for 3 min, followed by 40 cycles of denaturation at 95 C for 30 s, annealing at 58 C for 30 s and extension at 72 C for 30 s

doi: 10.1016/j.heliyon.2023.e19763 202 RodriguezJBystrynJC

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