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A portion of the tissue was preserved in an RNA stabilization solution for transcriptome sequencing and RNA extraction, while the remaining portion was fixed in 4% paraformaldehyde to facilitate the preparation of paraffin-embedded sections

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Further research indicated that the mechanism of these activities are partially related to IRF8-IFN-, IL-4-STAT6, Hedgehog, pERK1/2, MAPK, PI3K-Akt, NF-B, TNFR-c-Src-ERK1/2-c-Fos, Nrf2/HO-1, and HIF-1/VEGF signaling pathways (Figure 3 and Figure 4)

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Previously, the regulatory regions of DNA were considered as potential targets of epigenetic action for the AEDG peptide in the cell [2]

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