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Larsen et al., 1998) to provide a pool of mono-ubiquitin and regulate ubiquitin homeostasis

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albicans cells were fixed overnight at 4C in 2.5% glutaraldehyde (Sigma-Aldrich) on round coverslips (13 mm, Bionovo, Legnica, Poland) precoated with 0.001% poly-L-lysine (Sigma-Aldrich), and then washed five times with 0.1 M phosphate buffer, pH 7.2