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The Yalkowski model for solute solubility as a function of cosolvent-water mixtures (in this case ethanol) (3840) shows that 1% ethanol would only increase the solubility of niclosamide by 4% compared to pure buffer

doi: 10.1091/mbc.e06-10-0931 3 AlvesR.MotaS.SilvaS.CF

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If the solution is very viscous, add 3 units Benzonase per mL E.coli culture volume to the lysis buffer