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An overdose of vitamin B12 through diet is hardly possible and also not known, since the required transport protein IF (intrinsic factor) and absorption via the intestinal mucosa are two major barriers
Discard the vial when its expiration or recommended in-use period is reached
The serum cobalamin concentration can be measured by an automated chemiluminescence competitive binding immunoassay system but does not accurately reflect the real availability of cobalamin, because cobalamin-dependent metabolic reactions occur within cells
They can help address any concerns and guide you on the appropriate dosage
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This is a key variable to consider in experimental design
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