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(D) Type II PFPT involving several fungiform papillae scattered on the dorsal lingual surface

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Briefly, in a 200 l reaction volume, 5 g ml 1 recombinant GSTO1 in reaction buffer (100 mM Tris (pH 8.0), 1.5 mM EDTA and 1 mM DTT) was incubated with DMSO or different concentrations of inhibitors for 30 min at 37 C

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The compound demonstrates predictable pharmacokinetics with relatively rapid onset and offset characteristics, making it an excellent model for studying GH secretion dynamics