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Cells were harvested by centrifugation, resuspended in phosphate-buffered saline (PBS) buffer (140 mM NaCl, 2.7 mM KCl, 10 mM NaHPO, 1.8 mM KHPO, pH 7.4, and 10% glycerol), and lysed by sonication

And as mentioned before, fatigue and lethargy can also be a result of hormonal imbalances in women

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