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Compared with wild-type HEK293 cells, the EC 50 of vitamin K for the FSP1 KO cells increased only ~4-fold (27.0 nM vs 6.58 nM) (Fig

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In addition, it is considered quite easy to obtain a sufficient simulation of the placental barrier from placental choriocarcinoma cell lines, such as BeWo, Jar, and JEG-3, by permitting cells to construct both monolayers and multiple layers [35,36,37]

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For this experiment, we would expect there to be an increase in relative florescent units over time as the concentration of glycine increased