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Older adults are also at a higher risk of B12 deficiency due to decreased stomach acid levels, which are needed to separate B12 from protein in the diet for absorption

The most common side effect of this medicine is application site reactions such as burning, irritation, itching, and redness

On the other hand, it has been illustrated that homocysteine reduction could have favorable effects on migraine disability, severity, and frequency of attacks (54)

therefore, some level of safety in human subjects can be assumed

2.2 Culture and treatment of human umbilical vein endothelial cells (HUVECs) HUVECs were purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd

Guideline on bioanalytical method validation (EMEA/CHMP/EWP/192217/2009 rev