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Western blots Adult retinal tissue pooled from 1-3 fish (tissue from 2-6 eyes) were homogenized in Pierce RIPA Buffer (ThermoFisher) lysis reagent (1 L/mg tissue) supplemented with 1X Halt Protease Inhibitor Cocktail (ThermoFisher) using a sterile microcentrifuge pestle and cordless pestle motor (VWR)

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The semaglutide 2.4 mg (Wegovy) obesity treatment data [31] is presented alongside the graph to better illustrate how these biological influences may impact obesity treatment progression