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The number of living cells were counted by using an automated cell counter (Countess II, Thermo Fisher Scientific Inc.) after staining with trypan blue by pipetting for around 10 s

This ensures that researchers receive a reliable, high-integrity compound for their work, free from contaminants

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In the mouse study, the dietary dose levels were 0, 375, 750 and 1500 mg/kg diet using groups of 60 males and 60 females

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