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The signal was developed with the chromogen 3,3'-diaminobenzidine in a Bond-Max automatic slide stainer (Leica Biosystems Melbourne Pty Ltd., Melbourne, Australia)

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Additionally, research indicated that there may be a reduction in the expression of urea cycle enzymes, a possible re-establishment of nitrogen balance, and a potential modification or enhancement of nitrogen levels in different tissues

This case study allows us a privileged point of view, since each of the authors of this contribution is involved with a different role within this research network

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