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[1] TML is then hydroxylated into hydroxytrimethyllysine (HTML) by trimethyllysine dioxygenase (TMLD), requiring the presence of ascorbic acid and iron

After 2 days of culture (approximate doubling time of the cells), the cell monolayer was formed, and then the medium was replaced with DMEM without FBS for more than 12 h for serum starvation