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After 24 hpi, each well was washed, fixed with 4% Histofix (Roth), mounted with ProLong Gold antifade mountant (Thermo Fisher Scientific), and visualized at 64 magnification with an Axio Observer microscope (Carl Zeiss Microscopy) using bright field and fluorescence channels (596/621 nm)
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Acting in an intracrine manner, LTC4 then elicits nuclear translocation of NADPH oxidase 4 (NOX4), resulting in nuclear ROS accumulation, oxidative DNA damage and dsDNA breaks
In contrast, pathological stimulation involved cyclic stretch at 5% or 20% strain at 1 Hz frequency for 20 h in normoxia (21% O 2 ) and in hypoxia (1% O 2 ) combined with 20% cyclic stretch and exposure to NPs
1k, with AUC) as was food intake following the IPGTT and AL-fed blood glucose (Extended Data Fig
