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The concentrations of glucose and acetic acid were monitored by HPLC (LC-10AT, Shimadzu, Japan) using an Amines HPX-87H column (Bio-Rad, USA) and a refractive index detector (RID-10A, Shimadzu, Japan)

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combination injectable therapy should be considered as initial therapy for patients with a blood glucose of 300 mg/dL or higher, patients with an A1C of 10% or higher, and patients who are markedly symptomatic