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Secretion of glucagon-like peptide-1 in patients with type 2 diabetes mellitus: systematic review and meta-analyses of clinical studies

T84 cells (ATCC, CCL-248) were maintained in HAMs F12/DMEM (1:1) with L-glutamine (ThermoFisher) supplemented with 10% HI-FCS, 25 g/mL streptomycin and 25 U/mL penicillin (ThermoFisher)

doi: 10.1056/NEJMra2214275

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The ALP activity was determined by enzymatic colorimetric test 23