(v) reduced synapse support in ageing 151 (Fig
All catheters were flushed with phosphate-buffered saline (0.1 M, pH 7.4) approximately four hours before commencement of the experiments
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Liver and kidney function tests can identify any unexpected impacts on organ function
Following permeabilization (0.2% Triton-X100 in PBS, 10 min, RT), endogenous peroxidases were blocked by a 10 min incubation in a 3% H 2 O 2 solution, followed by a 1 h RT incubation in PBS with 2% bovine serum albumin (BSA, Amresco, Solon, OH)
Restraints 5.3.1
