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Truncated soluble His 6x tagged human FcRn lacking the transmembrane domain (hFcRn-His) was produced in a Baculovirus expression system and purified using a HisTrap HP column (Cytiva Life Sciences) 68,69

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Another unique property of REW is that human FcRn binding and cellular transport properties are enhanced for the four human IgG subclasses while their ability to engage C1q and FcRIIIa occurs in a subclass specific manner