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In addition, a specific membrane protein, Amuc_1100, exists on the outer membrane of A

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For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C

[PubMed] [Google Scholar] 126.Tanaka K.-I., Sugizaki T., Kanda Y., Tamura F., Niino T., Kawahara M

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However, for people who are already vulnerable to disordered eating, the appetite suppression these medications produce can, in some circumstances, interact with or reinforce difficult patterns around food