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This work was supported by the National Health and Medical Research Council of Australia (NHMRC) Project grants (1006141, 10305133, 1086887, and 1095215)

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HMEC1-hFcRn cells 63 were maintained in MCDB 131 medium (ThermoFisher) supplemented with 10% HI-FCS, 2 mM L-glutamine, 25 g/mL streptomycin, 25 U/mL penicillin, 10 ng/mL mouse epidermal growth factor (mEGFR) (Peprotech) and 1 g/mL hydrocortisone

On the third day, replace the medium with a fresh one containing M-CSF