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Step 7: Remove the syringe

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Cell layers were scrapped, extracted overnight with acid-based buffer (0.5 M acetic acid), and resulting pellets digested with 0.5 mg/ml pepsin (Sigma-Aldrich) in 10 mM HCl

Markers of pyroptosis and pulmonary vascular remodeling, as well as components of the C10:2/HSP47/NLRP3 axis, were evaluated using western blotting, ELISA, and biochemical assays

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