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Immunoblot Cells were lysed in protein lysis buffer (Cell Signaling) supplemented with Roche complete Protease Inhibitor Cocktail tablets (Sigma-aldrich)

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We demonstrated that Gln promotes proliferation by maintaining GSH-ROS balance, and refined the Gln/c-Myc/miR-29b-3p/GGCT regulatory axis, which links Gln availability to GGCT expression

Finally, ELISA analysis showed that L-carnosine markedly decreased the secretion of the pro-inflammatory cytokines IL-6 and TNF- following LPS stimulation (Figures 6G, H), indicating an anti-inflammatory effect