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Gould NS, Day BJ

Nat Med (2024)

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Analytical Method & Instrumentation Method:Reversed-Phase HPLC per USP Instrument:Agilent 1260 Infinity II HPLC System (SN: DE62244) Column:Agilent ZORBAX Eclipse Plus C18, 250 x 4.6 mm, 5 m (Lot: B80360) Mobile Phase A:0.1% Trifluoroacetic acid (TFA) in Milli-Q Water (18.2 Mcm) Mobile Phase B:0.1% TFA in Acetonitrile (HPLC Grade, Fisher Scientific) Gradient Program:0 min 5%B, 5 min 20%B, 20 min 60%B, 28 min 95%B, 30 min 95%B Flow Rate / Temp:1.0 mL/min / 30.0 0.5C (thermostatted column compartment) Detection:UV-DAD at 220 nm (bandwidth 4 nm, ref

This appears to be consistent with the observations made for exendin-4 under the same circumstances

Chung C et al (2023): New Practice Guidelines for Neuromuscular Blockade