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Glutamate homeostasis and neurotransmission play a major role in the onset of depression and anxiety

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We found a significant reduction in cathelicidin antimicrobial peptide (CAMP) (log2 fold = 3.743, P = 0.038) and placenta enriched 1 (PLAC1) (log2 fold = 7.071, P = 0.032) GP levels 1 week after 4-week intermittent fasting compared with the levels before 4-week intermittent fasting

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After 8 h of transfection, the medium was changed by fresh ECM containing 5% FBS and cells were harvested or used for further study in vitro after 48 h