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6 , e22572 (2011)

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New roles for dopamine D 2 and D 3 receptors in pancreatic beta cell insulin secretion

Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

You might notice it first in the gym, where workouts that used to leave you energized now leave you exhausted