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A notable rise in global prevalence of diabetes and obesity has accelerated product adoption in both developed and emerging markets

To ensure that most cells receive only one genetic perturbation, four 12-well plates at a cell density of 3 10 6 cells in 2 ml of DMEM/F12 medium per well were used for library transduction at a multiplicity of infection (MOI) of 0.3 to achieve a coverage of ~565 cells expressing each sgRNA

The gene responsible for PCD is SLC22A5

Holmes EW, Yong SL, Eiznhamer D, and Keshavarzian A (1998)

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But if the cells are already dead, the microglia form into a "jellyfish" blob (row C in the image) and try to plug up the holes and recycle the dead cells