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The absorbance of the released p- nitroanilide was measured every 2 min over 4 h at 405 nm using a Thermo Scientific Multiskan Ascent microplate reader

I administer B12 injections subcutaneously, which means right under the skin and not into the muscle

Brain-derived neurotrophic factor protects against cardiac dysfunction after myocardial infarction via a central nervous system-mediated pathway