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For SH-SY5Y/MYCN, the culture media was supplemented with 7.5 g/mL Blasticidin (Bio-Sciences) and 200 g/mL Geneticin (G418) (Thermo Scientific) for selection of the plasmid
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baumannii strains isolated from the blood of patients with bacteremia
Benefits depend on overall health, consistency of use, and proper dosing
Affinity purified protein samples were further resolved by size exclusion chromatography and assessed by SDS-PAGE and negative stain TEM before vitrification and cryo-EM imaging (Supplementary Fig