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Modulation of 5-S-cysteinyldopa formation by tyrosinase activity and intracellular thiols in human melanoma cells

Treatment of animals Four groups of Sprague-Dawley rats housed under the same conditions and fed on the same diet were used in this experiment

Briefly, 8000 cells were seeded per well, and then cultured in RPMI1640 medium which added 50 ng/ml EGF and 20 ng/ml bFGF for 1 week

We show for the first time that cellular proliferation specifically relates with nuclear, but not with total cellular GSH levels

Simultaneous measurement of foliar glutathione, -glutamylcysteine and amino acids by highperformance liquid chromatography: comparison with two other assay methods for glutathione

The expressed proteins of GSTP-silenced and control siRNA-treated cells on Day 4 after siRNA transfection were quantitatively compared