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Healthy cells have more of the reduced form than the oxidized form

To ensure harvesting of all white blood cells, the cells were centrifuged over a Ficoll density gradient at 2000 g for 10 min

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On the other hand, treatment of cancer cells with the anti-IGF-IR mAb cixutumumab paradoxically triggers cancer cells to produce IGF2, which in turn recruits macrophages and fibroblasts to promote angiogenesis and metastasis thereby hampering the efficacy of cixutumumab [190]

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Here's how it works, the benefits, and how it's used