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Immunoprecipitation and immunoblotting Cells were initially lysed on a rotation wheel in ice-cold RIPA buffer (50 mM Tris, 150 mM NaCl, pH 8.0, 0.5% deoxycholate, 1% NP-40, 0.1% SDS) for 1 h

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Enzyme replacement therapy clears GB3 deposits from a podocyte cell culture model of Fabry disease but fails to restore altered cellular signaling