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Increased liver weight, increased replicative DNA synthesis, and inhibition of gap-junction intercellular communication induced by a two-week treatment with diethlyhexyl phthalate or phenobarbital returned to control levels within one to four weeks following cessation of treatment (Isenberg et al

Evid Based Complement Altern Med

PMID: 11115795

Journal of Clinical Investigation, 63, 53-58

It is also a co-factor for GPX4-mediated lipid detoxification 35 , and GPX4 activity is necessary for the survival of several tissues, including the liver 36 and kidney 37

Furthermore, western blot analysis took place by initially preparing cell extracts in a lysis buffer followed by the standard method of ultrasonication