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These cells were then mounted in ProLong Glass antifade mounting medium (Life Technologies, USA), followed by treatment with fluorophore-conjugated goat anti-mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 and goat anti-rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 antibody and subsequent washing

Outcomes Co-primary endpoints were percentage change in body weight from baseline to week 104 and achievement of weight loss of at least 5% of baseline weight at week 104

To this end, high-fat-diet (HFD) feeding in mice has been shown to induce beta-cell-like changes in the electrical profile of a subset of alpha-cells (73)

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Periodic monitoring of liver fat provides a more comprehensive understanding of the effectiveness of obesity therapies