After consideration of public comments, we have adopted this clarification
Access this article Similar content being viewed by others References Correale M, Leopizzi A, Mallardi A, Ranieri A, Suriano MP, DAlessandro D, Tricarico L, Mazzeo P, Tucci S, Pastore G, Maulucci G, Di Biase M, Brunetti ND (2020) Switch to direct anticoagulants and improved endothelial function in patients with chronic heart failure and atrial fibrillation
Procedure Preheat spectrophotometer/microplate reader for 30 minutes, adjust the wavelength to 412 nm, set zero with distilled Preheat Reagent II in 37 (mammal cell) or 25 (other species) water bath for 30 Blank tube determination: take micro glass cuvette, add 20 L of distilled water, 140 L of Reagent II, 40 L of Reagent III in turn, mix well, place for 2 minutes, and measure 412 nm absorbance AB
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