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Congenital heart disease treatment may involve: A catheter procedure to place a plug into a defect

The pH of each sample was adjusted within the range of 7 to 9 using 014 pH strips (#13640516, Thermo Fisher Scientific) before subjecting them to digestion with mass spectrometry-graded trypsin (#90057, Thermo Fisher Scientific) at a 1:20 trypsin to protein (w/w) ratio

Altern Med Rev 2005;10(4):268-293

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Drug Saf., 6: 341-355

This curve can then be extrapolated to very high temperatures