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Plates were incubated at room temperature for 2h before measurement of the fluorescence using an EnVision Multimode Plate (Reader PerkinElmer Life and Analytical Sciences)

DPP-4 inhibitor plus SGLT-2 inhibitor as combination therapy for type 2 diabetes: from rationale to clinical aspects

First, we knocked down SIRT1 expression by Sirt1 RNA interference (i) using a lentivirus shRNA vector transfected into differentiated 3T3-L1 adipocytes