amino acids for glutathione synthesis Metabolism - an overview Glutathione metabolism. In the presence

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes

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Concurrently, mitochondrial ROS (mtROS) amplifies vascular dysfunction by oxidizing BH4, uncoupling endothelial eNOS, and reducing NO bioavailability while also activating pro-inflammatory pathways (e.g., NLRP3 inflammasome) and stimulating NOX activity, creating continuous oxidative damage (Penna and Pagliaro, 2025)

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