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"Adipose tissue quality in aging: how structural and functional aspects of adipose tissue impact skeletal muscle quality." Nutrients 11.11 (2019): 2553

Cell suspensions were passed through a 70 m and 40 m cell strainer (BD Bioscience), resuspended in 2.5 mL of 40% Percoll preparation (20 mL Percoll [GE Healthcare], 2.2 mL 10PBS, 27.8 mL HBSS without Ca 2+ and Mg 2+ [Sigma-Aldrich]) and layered onto 1.5 mL 60% Percoll (30 mL Percoll, 3.3 mL 10PBS, 16.7 mL HBSS)

Effects of Dietary Protein on Thyroid Axis Activity

The gut microbiota of the healthy people is mainly composed of Firmicutes and Bacteroidetes, followed by actinobacteria and verrucoides (32)

Reddy YNV, Carter RE, Obokata M, et al