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Second, animal models can identify mechanistic patterns but may not reflect human physiology

VilarGomez E., CalzadillaBertot L., WaiSun Wong V., et al., Fibrosis Severity as a Determinant of CauseSpecific Mortality in Patients With Advanced Nonalcoholic Fatty Liver Disease: A MultiNational Cohort Study, Gastroenterology 155, no

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most patients take it on an empty stomach 30 minutes before food

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After incubation with the appropriate antibodies for 30 min, erythrocytes were lysed with lysis buffer (BD FACS Lysing Solution, BD Biosciences, San Jose, USA) for 10 min on a shaker, followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and washing with HBS