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For IMR-5/75, the culture media was supplemented with 5 g/mL Blasticidin (Bio-Sciences) and 50 g/mL Zeocin (Thermo Scientific) for plasmid selection
For those learning the technique, training with a healthcare provider or using reliable equipment with built-in safety measures can reduce errors, prevent contamination, and improve overall confidence in the injection process
Hypokalaemia (low blood potassium) in the first weeks of loading-dose treatment in patients who started severely deficient
Importantly, animals were singly housed to eliminate the exchange and cross-contamination of intestinal microbiomes, a factor that potentially biases studies with group-housed animals by minimizing microbiome variations for co-housed animals within a cohort
Indeed, we sweat just as much during winter as we do during summer, thanks to dry indoor heating and extra layers of clothing