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After 30min of further incubation, the conversion of DCFH-DA to the fluorescent product DCF was measured by a confocal laser scanning microscope with excitation at 488nm and an emission at 525nm to determine the concentration of ROS in the samples

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However, a functional assignment of these residues remains difficult because of the absence of structures with transition-state analogues that allow the discrimination between the glutathione moieties from the GSSR substrate or GSH (Fig

10.1002/14651858.CD007176.pub2 29 BlaserH.DostertC.MakT

This toxicity is considered to be primarily inflicted by acetaldehyde, and possibly propagates redox cycling and catalytic generation of OS [7]

doi: 10.3389/fncel.2020.581191 Received 08 July 2020 Accepted 30 November 2020 Published 23 December 2020 Volume 14 - 2020 Edited by John Zhang, Loma Linda University, United States Reviewed by Hermona Soreq, Hebrew University of Jerusalem, Israel