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The cells were harvested by centrifugation at 3000 g for 10 min and then resuspended in MMA infiltration buffer (10 mM MES pH 5.7, 10 mM MgCl 2 , 100 M acetosyringone) to adjust the OD 600 to 0.6

DMF is prepared at 15 mg/ml in DMSO, a mouse of 30 g needs 1.5 mg (50 mg/kg), that is 100 L and dilute in 300 L PBS and intraperitoneally injected

This safeguard ensures the drug is used safely and only when appropriate

Peripheral T cell expansion predicts tumour infiltration and clinical response

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In the SURMOUNT-1 tirzepatide program, lean mass again made up a minority of total loss with fat mass falling more ( Jastreboff et al., NEJM 2022 )