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Tag-cleaved, SEC-purified Antigen A was chemically biotinylated following purification using the manufacturers protocol in the ThermoFisher EZ-Link NHS-PEG4-linked Biotinylation kit (Catalog Number: 21455)

GSH-edited spectra were collected from a 25 40 30 mm 3 voxel centered in the left dorsolateral prefrontal cortex (see Figure 1 for voxel position), with the MEGAPRESS method (echo time (TE) = 131 ms, repetition time (TR) = 1800 ms, 128 edit ON/OFF pairs)

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Lippi G, Montagnana M, Salvagno GL, Guidi GC, Targher G (2008) Prevalence of folic acid and vitamin B 12 deficiencies in patients with thyroid disorders