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Those most at risk include vegans and vegetarians, people with digestive conditions such as Crohn's disease, coeliac disease or IBS, those taking long-term metformin or proton pump inhibitors, older adults whose ability to absorb B12 through the gut declines naturally with age, those with pernicious anaemia, and anyone with a history of chronic alcohol use

Parenteral vs oral vitamin B12 in children with nutritional macrocytic anemia: a randomized controlled trial

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After 14 days of treatment, the mice were euthanized via cardiac injection of sodium pentobarbitone given at 35 mg/kg