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After treatment with 3% H 2 O 2 for 15 min to quench endogenous peroxidase activity, sections were incubated with horseradish peroxidase-conjugated secondary antibody (Histofine simple stain MAX PO

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Additionally, they found that bone marrow-derived monocytes from bleomycin-treated mice exhibit enhanced migration in vitro compared to control mouse monocytes and that in vivo treatment of the mice with all three CSD subregions reversed this enhanced migration

After washing with PBS (3 5 min), sections were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (1:500, Invitrogen) for 2 h at RT protected from light