Cultured cells were stimulated for 6 h with PMA (50 ng ml 1 ) (BioGems) and ionomycin (1 g ml 1 ) (BioGems) in the presence of brefeldin A (1 g ml 1 ) (GolgiPlug, BD Biosciences), stained for viability (Zombie UV Fixable Viability Dye (BioLegend)) and surface markers, fixed and permeabilized with 3.7% formaldehyde (Sigma) and 0.1% NP-40, respectively, and stained for intracellular cytokines
K., Heerschap, A., Henning, A., Hetherington, H
J Inherit Metab Dis , 34: 1732, 2011
P-III-NP, being the more challenging of the two analytes, has no validated approach for LC-MS analysis
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