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Following mutagenesis, the plasmid template was digested using the Dpn I restriction enzyme to eliminate template plasmids and the plasmid was then transformed into competent E
It involves inflammation, hormones, immune signaling, the gut, and the nervous system
The Bradford assay was used to determine protein concentration of cell extracts, from there samples with the same amount of protein were separated using SDS-PAGE technology and transferred onto a PVDF membrane
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