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The homogenate was filtered through a 100-M cell strainer and centrifuged at 1,000 g for 10 min at 4 C, then the nuclear pellet was washed once in nucleus preparation buffer followed by resuspension in PBS with 1% BSA and 0.2 U l Roche Protector RNase inhibitor

It is designed to treat genitourinary symptoms such as vaginal dryness and discomfort

Area under the receiver-operating characteristic (ROC) curve (AUROC) was then calculated to evaluate its classification performance using SPSS 22.0 (SPSS Inc., Chicago, IL, USA)

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[6] Yaskos protocol has undergone a number of adaptations over the years

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