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This poster from the US FDA: Characterization of the effect of primary human hepatocyte (PHH) lots on function in CN Bio Innovations Liver-Chip using acetaminophen, provides some useful insights into the effect of donor-to-donor variability in OOC assays

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The regulation of cellular metabolism by tumor suppressor p53

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In this environment, cysteines are, in general, oxidized to cystine and are no longer functional as a nucleophiles

This is called the amortal chamber