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Diabetologia 55 (9), 24452455

Slides containing retinal sections were deparaffinized by immersing the slides in various subsequent ethanol dilutions(100%, 95%,70% and 50%), then rinsed with phosphate-buffered saline, pH 7.4 (PBS, Quality Biological, 119-069-101), followed by incubation in blocking buffer containing 0.5% normal donkey serum (Abcam, ab7475), 0.5% bovine serum albumin (BSA, GoldBio, A-420-100), 0.1% Triton X-100 (Sigma, T8787-100) in PBS pH 7.4 for 2 h at room temperature 28

Our results suggest that both increased S -glutathionylation processes and suppressed de-glutathionylation reactions contribute to hepatic S -glutathionylation in NAFLD

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